Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Zhuang medicine Shandougen, which is the dry radix and rhizome of the plant Sophora tonkinensis from the Leguminosae family, is one of the most important and famous traditional Chinese medicine in Guangxi Zhuang Autonomous Region and also distributed in Yunnan and Guizhou provinces. According to the Chinese Pharmacopoeia 2020, shandougen is used for clearing heat, removing toxicity, relieving sore throat and detumescence. In order to develop and utilize the aerial parts of S. tonkinensis more reasonably and scientifically, the studies for chemical constituents and their tyrosinase inhibitory activities of the aerial parts of S. tonkinensis were performed. Some modern chromatographic methods for the separation of chemical constituents were carried out, such as high-performance liquid chromatography, sephadex LH-20 column chromatography, MCI gel CHP 20P column chromatography, silica gel column chromatography, and thin layer chromatography. The structures of the pure compounds were established on the basis of their spectroscopic data, including the spectra of HR-ESI-MS, 1H NMR and 13C NMR. In addition, the isolated compounds were evaluated for their tyrosinase inhibitory activity through modern pharmacological methods. The results were as follows: (1) Ten compounds were separated from the aerial parts of S. tonkinensis and their structures were identified as salicylic acid (1), 4-hydroxybenzoic acid (2), luteolin (3), 8-prenylkeamferol (4), quercetin (5), daidzein (6), formononetin-7-O-β-D-glucoside (7), formononetin (8), tectorigenin (9), and genistein (10). All these compounds were isolated from the aerial parts of S. tonkinensis for the first time; (2) Compounds 4, 7 and 9 showed strong inhibitory effects toward tyrosinase after the evaluation for their biological activity. Particularly, compound 4 showed the strongest inhibitory activity with the IC50 value of (1.58 ± 0.31) × 10-5 mol·L-1. Compound 4, 7 and 9 could be a potential tyrosinase inhibitor after the analysis of bioactivity results. The above result enriches the previous studies on the chemical constituents and biological activity of the aerial parts of S. tonkinensis, further providing the scientific basis for the development and utilization of the non-medicinal parts of this herb.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-05-28 Cooperative journals: 《广西植物》
Abstract： To study triterpenoids from the roots of Salvia bowleyana, the 95% ethanol extract was isolated and purified by means of various column chromatographic techniques, including macroporous resin D101, MCI, normal-phase silica column, ODS, and reversed-phase HPLC. Nine compounds were obtained, and their structures were elucidated using physicochemical properties and spectroscopic data as oleanolic acid (1), 2α,3α-dihydroxyolean-12-en-28-oic acid (2), ursolic acid (3), 2α,3α-dihydroxyurs-12-en-28-oic acid (4), 2α,3β-dihydroxyurs-12-en-28-oic acid (5), 2α,3β,19α-trihydroxyurs-12-en-28-oic acid (6), 2α,3β,24-trihydroxyurs-12-en-28-oic acid (7), psiguanin A (8), and n-hexadecane acid (9). Compounds 1-9 were isolated from this plant for the first time.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》
Abstract：为了明确展毛野牡丹的化学成分，运用Diaion HP20SS、MCI、Sephadex LH-20柱层析和反相高效液相色谱等方法对展毛野牡丹根和茎的醇提物分别进行分离纯化，从中分离得到11个化合物，经波谱数据的分析，鉴定了它们的结构。它们是4-羟基-3-甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷（1）、3,4-二羟基苯乙醇4-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷（2）、龙胆酸5-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷（3）、2,4,6-三甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷 (4)、甲基6-O-没食子酰基-β-D-吡喃葡萄糖苷（5）、乙基6-O-没食子酰基-β-D-吡喃葡萄糖苷（6）、6′-O-没食子酰基黑樱苷（7）、没食子酸甲酯（8）、没食子酸乙酯（9）、2,6-二甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷（10）、 2-甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷（11）。所有化合物均为首次从展毛野牡丹中分离得到，化合物2-7、10和11为首次从该属植物中分离得到。
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